Based on the successful infinite 200 series, tecan has developed the infinite 200 pro, with new enhancements, like the automated adjustable zfocusing in the infinite m200 pro. Lansoprazole is an antituberculous prodrug targeting. Measurements were performed in a microplate reader tecan, infinite m200. Novel bioluminescent cell metabolism assays integration. Tecan infinite 200 instructions for use manual pdf download. To determine the variation due to the time it takes to measure the assay plate on each plate reader, the glomax. Longlife led technology provides maintenancefree operation, with the readers outstanding reliability demonstrated by its unique benchmark mean time to first failure value. Characterization of human duodenal fluids in fasted and. I am using tecan infinite m pro multi detection plate reader to study of protein and dna binding. Stay notified of promega events, products and news. Tecan infinite 200 instructions for use manual pdf. Absorbance was read at the wavelength of 570 nm using a microplate reader tecan infinite m200, austria. With the infinite f200 pro, tecan offers an affordable alternative to costintensive laserbased alpha detection systems.
None of the equipment posted for sale on is owned by medwow, should you have any questions regarding a specific item, please direct them to the appropriate seller by making use of the available communication channels on the items page. Add one tungsten ball to the lyophilized leaf tissue 10 to 40 mg or dry seeds 10 seeds 300 mg in 2ml microfuge tube. Frontiers multiparametric profiling for identification. Hpv16 e5 mediates resistance to pdl1 blockade and can be. Absorbance 230 nm is measured from transparent 96 or 384 well plates from the bottom while fluorescence standard. We recorded the optical density of these mixtures at different time periods via a microplate reader tecan infinite m200. Infinite 200 download tecan for noncontact dispensing of test compound and the tecan infinite m200 pro with without the need for any manual range from cytotoxicity. Infinite m200 pro the tecan journal is published several times a year, and contains articles featuring users of tecan instruments, as well as information about latest products and global tecan activities.
The assay was used in a 96well format with a detection limit of 0. Applications and assays that can include dnarna quantifications. Infinite 200 pro manual laboratory of biomimetic engineering. The company specializes in the development, production and distribution of automated workflow solutions for laboratories in the life sciences sector. Tecan infinite m pro microplate reader shared equipment. Minimum inhibitory concentration mic was performed using broth microdilution plate. Other articles in this issue show the wide range of applications for which tecan products are being used, including vaccine development, structural analysis, diagnostic testing, and studies of. Suitable for light sensitive reagents, and can be sealed for convenient reagent transport. We provide a number of innovative, offtheshelf solutions that are specifically tailored to applications in life sciences and diagnostics. The tecan infinite 200 is a computer controlled plate reader capable of detecting uvvis light, fluorescence, and luminescence.
Capture antibody was incubated on a 96well plate overnight. Tecan infinite 200 microplate fluorescence reader with tecan. With magellan, comprehensive reader control and data analysis software, the infinite f50 is ideal for a variety of elisa applications. Before a tecan fse carries out any work on the instrument, or before the instrument is returned to tecan, the owner of the instrument must confirm in writing that the decontamination has been performed properly and in accordance with good laboratory practice guidelines. Tecan infinite m200 microplate reader tecan us, inc. Tecan infinite f200 pro microplate reader manufacturer. The pipeting was performed by a tecan freedom evo automated liquid handler equipped with eight fixed ptfecoated tips, with a robotic arm to transfer plates to the tecan infinite m200 pro multimode plate reader. Quantification of each sample was performed using a standard curve of known concentrations. Wells were blocked for 1 h then incubated overnight with. It is the policy of tecan austria gmbh to improve products as new techniques and components become available. A special conical design allows maximum reagent recovery. The contents of this manual are the property of tecan austria gmbh and. Tecans infinite mm pro and infinite f500 have received the lanthascreen certified plus designation and tecans infinite f200200 pro have received the lanthascreen certified designation from life technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. The tecan infinite m200 pro microplate reader has a modular design allowing users to customize their machines to suit their needs and changing applications.
Infinite 200 laboratory equipment pdf manual download. Inflammationtargeting polymeric nanoparticles deliver. Biologically relevant bile salts tc, tcdc, tdc, gc, gcdc, and gdc at concentrations of 10 mm. This note describes the use of the fluorescence microplate reader tecan infinite f200 pro or m200 pro, for detection of as lias 20 pg dsdna in a 200. Tecan life sciences home tecan uses cookies to improve our website. If you need to run for an extended amount of time e. Tecan s engineers and scientists are committed to delivering the highest standards of quality by focusing on the details, ensuring that your data is true and consistent, empowering your discovery. This standard configuration is equipped with 405, 450, 492, and 620 nm filters ideal for photometric based assays in visrange of the spectrum.
How does one determine the reference polarization values. Wo2015002490a1 pctkr2014005975 kr2014005975w wo2015002490a1 wo 2015002490 a1 wo2015002490 a1 wo 2015002490a1 kr 2014005975 w kr2014005975 w kr 2014005975w wo 2015002490 a1 wo2015002490 a1 wo 2015002490a1 authority wo wipo pct prior art keywords photocatalytic upconverting nanoparticles metal oxide coated complex prior art date 20703. Building on the success of the infinite 200 series, tecan has developed the infinite 200 pro, with new enhancements that cater to the needs of todays scientists. The infinite 200 series is compatible with tecans new nanoquant.
Frontiers construction and characterization of synthetic. This plate reader accommodates 6 to 384well plates, with the option to ad modules to accommodate cuvettes, and pcr plates. View online or download tecan infinite 200 instructions for use manual. The mycorrhiza helper bacterium mhb pseudomonas fluorescens bbc6r8 promotes the ectomycorrhizal symbiosis between douglas fir roots and laccaria bicolor. Tweaking fluorescence scans guidelines to recording excitation and emission spectra on the infinite m200 pro and infinite m pro introduction the infinite m200 pro and infinite m pro are tecans latest generation of quad4 monochromatorsbased multimode microplate readers, packed with. Infinite, icontroltm, gcmtm, magellantm, nanoquant platetm,tecan and the tecan logo are. Not approved for human or veterinary use, for application to humans or animals, or for use in clinical or in vitro diagnostic procedures. The contents of this manual are the property of tecan austria gmbh and are not. Dec 08, 2010 a tecan uj infinite 200 pro mikroplate leolvaso csaladjanak hazai reklamfilmje. Conditioned medium was analyzed for ffa and glycerol content by commercial kits wako chemicals and sigmaaldrich, respectively. The system features fullspectrum absorbance and fluorescence emission detection, in situ optogenetic. Infinite f50 absorbance microplate reader from tecan. Microplate readers are foundational instruments in experimental biology and bioengineering that enable multiplexed spectrophotometric measurements. Tecans icontrol software kinetic measurement basics.
A simple, sensitive system to measure blockade of immune. Tecan offers compact, benchtop units for mass spec sample prep workflows, enabling applications such as analyte enrichment and sample cleanup to be performed under positive pressure. The infinite f50 is a validated and compact absorbance microplate reader for elisa assays. Tecans engineers and scientists are committed to delivering the highest standards of quality by focusing on the details, ensuring that your. Infinite 200 pro can provide a full range of leading detection methods in one easytouse modular instrument. How does one determine the reference polarization values for fam fluorophore in gfactor calculation. Emission 280850nm, fluorescence polarization excitation led.
Tecan infinite 200 pro instructions for use manual pdf download. Measurements were performed using a microplate reader tecan, infinite m200 in accordance to manufacturer instructions. Tecan infinite m200 pro absorbance microplate reader. Kinetic fluorescence measurements were performed for 10 min tecan infinite m200 multiplate reader. Dna extraction and sequencing for 16s rrna amplicon analysis. Tecan infinite f500 university of wisconsinmadison. Tecan pure purity level tested and certified to be free of human dna, rnase, dnase and pcr inhibitors making them ideal for molecular diagnostics, forensics and genomics research applications. Introduction a multimode microplate reader located at the main laboratory of ibec at the helix building is available for use by all ibec researchers. The absorbance at 450 nm was measured using a tecan microplate reader tecan infinite m200 pro, austria. Manufacturer specifications infinite f200 pro, tecan please note. Human thioredoxin 2 deficiency impairs mitochondrial redox. The infinite m200 pro from tecan can provide a full range of leading detection methods. Data were analysed against the linear portion of the generated standard curve. Substrate fluxes in brown adipocytes upon adrenergic.
The tecan infinite m pro is a computer controlled 6 to 1536 well plate reader capable of detecting absorbance 230 nm, fluorescence excitation 230850nm. Start working with magellan note the difference between plate definition files with. Tecan austria infinite m200 nanoquant quad4 monochromators. The transcriptic common lab environment tcle benjamin miles1, jimmy sastra1, matthew wong1. The infinite 200 pro is an easytouse plate reader family that offers affordable, high performance detection solutions in absorbance, fluroescence and. Positive pressure workstations smart sample preparation.
Application guide for tecan plate washers your easy way to optimized results 21 cfr part 11 9879ec ivdd 2. Infinite f50 absorbance microplate reader from tecan trading. In addition, the wells with only culture medium only were used as the background. A high throughput dna extraction method with high yield. For the bmg and tecan instruments, multiple gain settings were tested to determine the optimal setting. Tecans engineers and scientists are committed to delivering the highest standards of quality by focusing on the details, ensuring that your data is true and consistent, empowering your discovery. The cell viability was calculated according to the following formula. Many of these systems have been developed in collaboration with our partners, integrating our liquid handling and detection technologies with widely used kits. Assessment of the efficacy of a grape seed waste in. The infinite f50 is an eightchannel absorbance microplate reader offering rapid, accurate and reproducible measurements. The zposition and gain were optimized using the icontrol software tool and kept constant throughout the analysis for a given agncdna concentration.
If you are using an infinite m200 with cuvette option you can define. View and download tecan infinite 200 instructions for use manual online. Pseudomonas fluorescens bbc6r8 type iii secretion mutants. Cytotoxic and antioxidant effects of antimalarial herbal. Cells treated with npfree medium were used as the control group. Optical system of the absorbance module of infinite. Tecan infinite device driver quick reference about this document 2 tecan infinite device driver quick reference about this document what this guide covers the tecan infinite is a series of microplate reader models that can be integrated into a lab automation system that is controlled by the vworks software. In this note we describe the easy implementation of tecan. This technical note describes the relevant instrument parameters to acquire optimal fluorescence excitation and emission spectra on the infinite m200 pro. The software magellan is capable of kinetic reads and. The eschatological idea, as download range 4 m200 perkins manual pdf follows from the above that enlightens positivist jump function. The mtt assay spectrophotometrically measures the purplecoloured formazan resulting from the reduction of the yellow tetrazolium salt by metabolically active cells. Tecan austria gmbh therefore reserves the right to change specifications at any time with verification, validation, and appropriate approvals.
The 50% reduction in cell number relative to the control ic 50 was established by extrapolation from the graph of experimental data. Page 4 of 4 these products are for research use only. Infinite 200 pro laboratory equipment pdf manual download. Using infinite 200 nanoquant for absorbancebased protein.
Then, purple formazan crystals were dissolved in lysis buffer 4 mmoll hcl and 0. Infinite m200 pro multimode microplate reader from tecan reservations and usage policy 1. Unusual flavones from primula macrocalyx as inhibitors of. Ex 230 600 nm, em 330 600 nm is measured from the top. After 1 h at room temperature, fluorescence was measured in a tecan infinite m200 plate reader excitation 570 nm and emission 590 nm.
The two racks must hold equal number of tubes for balance. Step 3 select the zposition tecan infinite m200 provides the zposition adjustment to achieve best optical reading. The adjustable zfocus for fi top measurements, which offers equally high sensitivity for all plate formats, helping to implement assay miniaturization. Tecan reader device driver user guide 2 who should read this guide. The infinite f50 is tecans nextgeneration, 8channel absorbance microplate reader that provides accurate, reproducible and fast measurements out of a compact, innovative design.
All turnon fluorescence study was carried out on a microplate reader tecan infinite m200. Microplate reader tecan infinite m200 pro usescience. This example is relevant to repeat measurements of the entire plate aka platewise readings. Standard abts elisa peprotech, usa was performed according to manufacturers protocol. This video explains how to set up a simple kinetic reading in icontrol software. The infinite 200 pro offers flexible, scaleable detection solutions for a wide range of assays, using monochromator and filterbased technologies. Protein is 40 kda, and 15 bp dna is labeled with 5 cy3 on one strand. To select the zposition, an optimiser plate will be used with one well containing positive control solution material. View and download tecan infinite 200 pro instructions for use manual online. The infinite 200 pro is a userfriendly and affordable multimode reader, designed to. To enhance their accessibility, we here report the design, construction, validation, and benchmarking of an opensource microplate reader. Using infinite 200 nanoquant for absorbancebased protein detection in smallvolume. Tecan infinite 200 pro instructions for use manual pdf. Novel bioluminescent cell metabolism assays integration with hp d300 digital dispenser and tecan gas controlled module equipped infinite m200 pro reader.
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